Blue Heron Biotech can provide a wide variety of mutations, including single nucleotide polymorphisms (SNPs), complex block variants, splice variants, as well as library variants. Library pricing is based on sequence complexity and the constructs degree of similarity to the base sequence. Our best effort will be made to re-use the base DNA template you ordered for its variant build and pass the savings to you.
There are many potential options for your variant project. It may be that you would like the same sequence with different cloning ends for shuttling to another vector with different restriction enzymes in the multiple cloning sites (MCS). We can also offer discounts for truncations of the original master sequence. SNP’s are the simplest form of variants and will receive our very best discounted pricing.
Library Delivered as Sequence Verified Individual Clones
As with simple variants, the pricing of variant libraries is based on sequence complexity and the constructs degree of similarity to the base sequence. Additional discounts are available for libraries of more than 10 constructs. Your project may require numerous changes to the base gene at several locations throughout the sequence. We can also exchange or shuffle large regions or domains within the sequence.
Library Delivered as Mixed Fragments or Cloned Plasmid
Applications of Variant Genes
- Substitutions, Deletions or Insertions
- Comprehensive codon substitutions
- Variant clone sets with thousands of substitutions
- Variant clone libraries