Blue Heron’s GeneMaker® is a unique, robust, automated design and synthesis platform. Unlike commonly used PCR synthesis methods, GeneMaker® incorporates multiple novel technologies to first design and later synthesize each DNA sequence. The GeneMaker® manufacturing process is fully automated, so Eurofins Genomics Blue Heron can scale rapidly according to your needs. With a suite of online tools and a dedicated customer service team, ordering gene synthesis has never been easier.
Online Ordering & Quote Generation
Download Sequence Data
Cas9 gRNA Design Tool
Access Archived Vector Collection
Genemaker® is a fully automated gene synthesis platform that covers the entire synthesis process.
Ordering begins on our dedicated secure website. Simply log in and enter your desired sequence. Then our proprietary algorithm will optimize the best build for your gene. Our build strategies strive to reduce complexity and simultaneously promote protein expression.
Oligonucleotides are made in house to the highest quality standards. Eurofins Genomics Blue Heron’s oligo synthesis capacity eliminates the need to outsource any synthesis. This allows savings and efficiency to be passed on to our customers.
Depending on the complexity and size of your project, we have a variety of assembly techniques. Our proprietary build techniques reach beyond common PCR synthesis methods utilizing solution and solid substrate technologies.
Your gene is then cloned into the vector of your choice and sequence verified to ensure 100% accuracy. Each step in the Genemaker® process is held to strict quality control standards, allowing for precise deliverables as well as quick turnaround time.
With Eurofins Genomics Blue Heron Biotech’s GeneMaker, you can customize your gene synthesis order to any specifications. We offer Plasmid Preparations, Entotoxin-free options, Glycerol Stocks, Quality Assurance Assistance, and free archiving or genes and vectors. Ordering an additional shipment of your construct is always fast and cost effective.
Secure Gene Synthesis Orders
Multiple Build Strategies
Oligos are synthesized and assembled based on the instructions from the GeneMaker database. Eurofins Genomics Blue Heron builds oligos using cutting-edge automated technology. These oligos are then hybridized into duplexes which and assembled into subtargets using the best build strategy.
Every step of the synthesis process takes place within our labs. Eurofins Genomics Blue Heron Biotech maintains the highest levels of synthesis expertise, eliminating the need to outsource oligo production. Only oligos meeting the highest quality standards are used in this process, ensuring quality, consistency, and accuracy are maintained.
Cloning and Sequence Verification
Genes are cloned into either a standard vector (e.g., pUC derivatives) or a customer-provided vector. Eurofins Genomics Blue Heron provides free archiving of genes and vectors, allowing for fast and cost effective re-shipments of any construct. GeneMaker facilitates an easy re-order process of any gene in any vector by providing each customer a personal archived vector collection.
Every sequence synthesized is sequenced using Gold Standard methods of double-stranded, primer walk, Big Dye methodology. Eurofins Genomics Blue Heron customers receive the synthesized gene sequence exactly as ordered.
- 3-5 ug purified plasmid DNA
- Bacterial strain containing the plasmid
- Sequencing and trace data posted electronically
- Quick turnaround time
Customized Gene Synthesis
- 100ug, 500ug, or mg Plasmid Preparations
- Endotoxin-Free Options
- Express Service on any Gene
- Order Linear Fragments
- Glycerol Stock
- Additional Quality Control Measures and Documentation