We can clone into any vector in E. coli
Eurofins Genomics Blue Heron provides several vector options for your convenience. With twenty years of gene synthesis and custom cloning experience, we can clone into any vector. From expression vectors, to CRISPR guide vectors and lentiviral vectors, we will deliver. Our vector choices include Blue Heron’s standard vectors, any custom vector you provide, a selection of expression vectors, or building your own vector.
Save valuable time! By utilizing Eurofins Genomics Blue Heron for synthesis and subcloning, customers save time and costly reagents that would have otherwise been used for cloning. Our efficient cloning services ensure that custom cloning adds minimal time to the production of your order. Eurofins Genomics Blue Heron provides free archiving of vectors and synthesis products, allowing for a quick and affordable option for reshipment, or subsequent cloning into your vector. As with all of our services, express service is available for all subcloning projects.
Selection of Vectors
|Vector Name||Size||Antibiotic Resistance||Multiple cloning site||Sequence||Map||Copy in E. Coli|
|Blue Heron pUCAmp||3150 bp||Ampicillin||Yes||Sequence||Map||High|
|Blue Heron pUCKan||3284 bp||Kanamycin||Yes||Sequence||Map||High|
|Blue Heron pUCAmpMinusMCS||3171 bp||Ampicillin||No||Sequence||Map||High|
|Blue Heron pUCkanMinusMCS||3305 bp||Kanamycin||No||Sequence||Map||High|
OriGene Vector Selection
- Epitope tags (MYC, DDK, etc.) for protein purification detection
- Fluorescent tags (GFP, RFP, YFP, etc.) for protein visualization
- Various drug markers (neomycin, puromycin, etc.) for stable cell line establishment
- E. coli expression vectors
- Custom requests
Provide Your Own Vector
Eurofins Genomics Blue Heron will clone into any vector provided by our customers. To order cloning into your vector of choice, simply log in to the secure website and select Set-up My Vector. This tool allows you to submit your vector sequence information and store it in your account. Any vectors stored in your account will be available to you in your list of vectors when submitting an order for gene synthesis.
Build or Modify your Vector
Eurofins Genomics Blue Heron’s gene synthesis capacity allows us to synthesize almost any sequence, no matter how complex. We can synthesize and clone your choice of vector modifications including promoter sequences, cloning sites, reporter genes, fluorescent tags, genetic markers, antibiotic resistance, and protein purification tags.
Cloning unique features into your starting vector can be a time consuming and costly process. Eurofins Genomics Blue Heron’s gene synthesis expertise enables us to synthesize and clone any desired feature into any starting vector. Once your modification is complete, Blue Heron will ship you your sequence verified plasmid and archive it at our secure site in Bothell, Washington. Your vector will be available for use in any subsequent cloning projects. Each of your unique vector features are also securely stored for use in future projects. Our efficiency allows you to perform a number of vector modifications quickly and easily. We offer simple, flat rate pricing, secure online ordering, and an express service option for every order.