GeneMaker® Cloned Variants
Blue Heron can provide a wide variety of mutations and splice variants, SNPs, or complex block variants. Just like de-novo synthesis, all variants are delivered sequence-verified and individually cloned.
If your variant project is more complex than just a few genes, Blue Heron will work with you to find the best approach to obtain the full set of clones as economically as possible. For large numbers of variants, our high-throughput process builds sets of variants in parallel for quick delivery regardless of the number or complexity of variants required.
If your requirements are specialized or unusual, we will work with you to provide what you need. Our GeneMaker technology maximizes re-use of de-novo regions from the "base gene," minimizing the amount of de-novo synthesis required for each variant. Also, we have developed specialized techniques that allow complete codon substitution at one or hundreds of regions, delivering a complete set of ten's, hundred's, or thousand's of sequence-verified, individually-cloned variant genes.
Examples of what you can do with variant genes:
- Domain swaps
- Heavily mutated promoter regions
- Fusion proteins
- Tagged proteins
- Knockout constructs
- Deletions
- Insertions
- Substitutions
- Alternative splice forms
- Comprehensive codon substitutions
- Variant clone sets with thousands of substitutions
- Variant clone libraries