Custom Cloning

Blue Heron can clone your ordered sequence into the vector you need to get your project completed as quickly and efficiently as possible. Choices include standard Blue Heron vectors, a selection of third-party and proprietary vectors, or customer-supplied vectors.

Vectors with no additional charge:

  • Blue Heron pUCminusMCS, which lacks a multiple cloning site
  • Blue Heron pUC, a pUC119 derivative containing a multiple cloning sites

Customer-provided vectors:

  • Customer proprietary vectors — Customer can provide their own vector and Blue Heron will store it at their site to make it available for ongoing cloning projects. One-time check-in process includes a small additional charge.
  • The pET vectors — Customer's license from Brookhaven National Labs must include third-party cloning.
  • Expression vectors
  • Specialized vectors for large projects can be used to minimize overall cost - Ask!

OriGene-provided vectors:

OriGene has developed a novel pCMV6-based, versatile protein expression vector system. The PrecisonShuttle system uses a simple 'cut-and-paste' procedure to subclone any gene inserts from any vector into any of 60 destination vectors. The destination vectors offer choices of:

• Epitope tags for protein purification detection
• Fluorescent protein tags for protein visualization
• Various drug marker for stable line establishment
• Inducible expression (pTUNE)
• E.coli expression vectors

Please contact a Blue Heron Bio representative regarding your specific cloning needs or questions on how to order: customerservice@blueheronbio.com or (425) 368-4060.